Development of Novel Correlative Light and Electron Microscopy Linkage System Using Silicon Nitride Film
Microscopy(2019)
摘要
In this study, we investigated the optical properties of a silicon nitride (SiN) film. The thin SiN film (30 nm thick) exhibited good light transmittance and little autofluorescence and could be used as a microscope slide for optical microscopy (OM). In addition, we developed a novel correlative light and electron microscopy (CLEM) that combines OM with transmission electron microscopy (TEM) using an SiN thin film. In this system, CLEM was performed by replacing a detachable retainer with a holder for TEM and an adaptor for OM. The advantage of this method is that the same specimens can be sequentially observed using suitable OM and TEM.
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关键词
correlative microscopy,SiN film,OM-TEM integration,exchangeable retainer,TEM holder
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