Morpholino Oligonucleotide Cross-Linked Hydrogels As Portable Optical Oligonucleotide Biosensors
ACS sensors(2018)
摘要
Morpholino Oligonucleotides (MOs), an uncharged DNA analogue, are functionalized with an acrylamide moiety and incorporated into polymer hydrogels as responsive cross-links for microRNA sequence detection. The MO cross links can be selectively cleaved by a short target analyte single-stranded DNA (ssDNA) sequence based on microRNA, inducing a distinct swelling response measured optically. The MO cross-links offer significant improvement over DNA based systems through improved thermal stability, no salt requirement and 1000-fold improved sensitivity over a comparative biosensor, facilitating a wider range of sensing conditions. Analysis was also achieved using a mobile phone camera, demonstrating portability.
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关键词
morpholino oligonucleotide,oligonucleotide sensor,biosensor,hydrogel,microRNA
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