Combination of ribosome display and next generation sequencing as a powerful method for identification of affibody binders against β-lactamase CTX-M15.

Priscillia Lagoutte,Adrien Lugari,Céline Elie,Supanee Potisopon,Stéphanie Donnat,Charlotte Mignon,Natacha Mariano, Alain Troesch,Bettina Werle,Gustavo Stadthagen

New Biotechnology（2019）

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摘要

•Coupling ribosome display to NGS allowed identifying specific anti-CTX-M15 affibodies.•Affibody ligand is able to detect CTX-M15 in simple immune-based tests.•Affibody binding leads to a decrease of CTX-M15 substrate affinity.

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关键词

AA,nM,μM,CTX-M,ESBL,ELISA,RBS,NGS,IVTT,RD

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