Imaging Of Biomolecular Nmr Signals Amplified By Reversible Exchange With Parahydrogen Inside An Mri Scanner
JOURNAL OF PHYSICAL CHEMISTRY C(2017)
摘要
The signal amplification by reversible exchange (SABRE) technique employs exchange with singlet-state parahydrogen to efficiently generate high levels of nuclear spin polarization. Spontaneous SABRE has been shown previously to be efficient in the milli-Tesla and micro-Tesla regimes. We have recently demonstrated that high-field SABRE is also possible, where proton sites of molecules that are able to reversibly coordinate to a metal center can be hyperpolarized directly within high field magnets, potentially offering the convenience of in situ hyper-polarization-based spectroscopy and imaging without sample shuttling. Here, we show efficient polarization transfer from parahydrogen (para-H-2) to the N-15 atoms of imidazole-N-15(2) and nicotinamide-N-15 achieved via high-field SABRE (HF-SABRE). Spontaneous transfer of spin order from the para-H-2 protons to N-15 atoms at the high magnetic field of an MRI scanner allows one not only to record enhanced N-15 NMR spectra of in situ hyperpolarized biomolecules but also to perform imaging using conventional MRI sequences. 2D N-15 MRI of high-field SABRE-hyperpolarized imidazole with spatial resolution of 0.3 x 0.3 mm(2) at 9.4 T magnetic field and a high signal-to-noise ratio (SNR) of similar to 99 was demonstrated. We show that H-1 MRI of in situ HF-SABRE hyperpolarized biomolecules (e.g., imidazole-N-15(2)) is also feasible. Taken together, these results show that heteronuclear (N-15) and H-1 spectroscopic detection and imaging of high-field-SABRE-hyperpolarized molecules are promising tools for a number of emerging applications.
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