Lactobacillus Bulgaricus Or Lactobacillus Rhamnosus Suppresses Nf-B Signaling Pathway And Protects Against Afb(1)-Induced Hepatitis: A Novel Potential Preventive Strategy For Aflatoxicosis?

Aflatoxin B-1 (AFB(1)), a mycotoxin found in food and feed, is immunotoxic to animals and poses significant threat to the food industry and animal production. The primary target of AFB(1) is the liver. To overcome aflatoxin toxicity, probiotic-mediated detoxification has been proposed. In the present study, to investigate the protective effects and molecular mechanisms of Lactobacillus bulgaricus or Lactobacillus rhamnosus against liver inflammatory responses to AFB(1), mice were administered with AFB(1) (300 g/kg) and/or Lactobacillus intragastrically for 8 weeks. AML12 cells were cultured and treated with AFB(1), BAY 11-7082 (an NF-B inhibitor), and different concentrations of L. bulgaricus or L. rhamnosus. The body weight, liver index, histopathological changes, biochemical indices, cytokines, cytotoxicity, and activation of the NF-B signaling pathway were measured. AFB(1) exposure caused changes in liver histopathology and biochemical functions, altered inflammatory response, and activated the NF-B pathway. Supplementation of L. bulgaricus or L. rhamnosus significantly prevented AFB(1)-induced liver injury and alleviated histopathological changes and inflammatory response by decreasing NF-B p65 expression. The results of in vitro experiments revealed that L.rhamnosus evidently protected against AFB(1)-induced inflammatory response and decreased NF-B p65 expression when compared with L. bulgaricus. These findings indicated that AFB(1) exposure can cause inflammatory response by inducing hepatic injury, and supplementation of L. bulgaricus or L. rhamnosus can produce significant protective effect against AFB(1)-induced liver damage and inflammatory response by regulating the activation of the NF-B signaling pathway.