Bead preparation protocol v1

Peter H L Krijger,Tim A Hoek,Sanne Boersma,Lieke I P M Donders, Maaike M C Broeders,Mark Pieterse, Pim W Toonen, Ive Logister,Bram M P Verhagen,Marjon J A M Verstegen,Thomas W van Ravesteyn,Rene J T M Roymans,Francesca Mattiroli,Jo Vandesompele,Monique Nijhuis, Stefan Meijer, Anton van Weert, Edwin Dekker,Fred J Dom, Rob Ruijtenbeek, Lieven B J van der Velden, Jeroen H B van de Bovenkamp, Martijn Bosch,Wouter de Laat,Marvin E Tanenbaum

semanticscholar（2021）

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摘要

STRIP is a start-to-end streamlined and automated procedure for COVID-19 testing, centering on a single Tecan Fluent liquid-handling robot that can process over 14,000 samples per day. Here we describe the protocol to prepare the magnetic beads that are currently used in STRIP. Note, in this protocol we use Sera-Mag SpeedBead Carboxylate-Modified Magnetic Particles (Hydrophobic) from Cytivia, but other beads such as SeraSil Mag 400 beads (Cytiva) can be used as substitutes.

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