BLADE-ON-PETIOLE genes temporally and developmentally regulate the sheath to blade ratio of rice leaves

Axis formation is a fundamental issue in developmental biology. Axis formation and patterning in plant leaves is crucial for morphology and crop productivity. Here, we reveal the basis of proximal-distal patterning in rice leaves, which consist of a proximal sheath, a distal blade, and boundary organs formed between these two regions. Analysis of the three rice homologs of the Arabidopsis BLADE-ON-PETIOLE1 ( BOP1 ) gene indicates that OsBOPs activate proximal sheath differentiation and suppress distal blade differentiation. Temporal expression changes of OsBOPs are responsible for the developmental changes in the sheath:blade ratio. We further identify that the change in the sheath:blade ratio during the juvenile phase is controlled by the miR156/SPL pathway, which modifies the level and pattern of expression of OsBOPs . OsBOPs are also essential for differentiation of the boundary organs. We propose that OsBOPs , the main regulators of proximal-distal patterning, control temporal changes in the sheath:blade ratio of rice leaves.