Prognostic Characterization of Higher-Grade Meningiomas: A Histopathological Score to Predict Progression and Outcome.

Higher-grade meningiomas (WHO grade II and III) represent a diagnostic and prognostic challenge. We assessed the pathological and molecular characteristics of 94 higher-grade meningiomas (85 grade II, 9 grade III) to identify novel prognostic parameters. Higher mitotic count (p=0.018), diffuse (50%) prominent nucleoli (p<0.001), and sheeting (p<0.001) were associated with recurrence. Lower SSTR2a-positive cells median rate (p=0.048) and TERT promoter mutations (p=0.014) were associated with recurrence and patient death, respectively; further analyses did not identify other outcome associations. Presence of Ki67 hot spots was associated with a shorter progression-free survival (PFS), independently of WHO grade at multivariate analysis (HR=3.35, p=0.008). Necrosis was related to a poorer overall survival (OS) at univariate (focal: HR=4.55, p=0.041 and diffuse: HR=7.38, p=0.020) and Kaplan-Meier analyses. A prognostic score was designed based on previous results: Presence of diffuse (50%) prominent nucleoli (0/1 point), diffuse (50%) sheeting (0/1 point), focal (<50%) or diffuse (50%) necrosis (0/1/2 points), and Ki67 hot spots (0/1 point). A total score 4 predicted poorer PFS and OS by Kaplan-Meier (PFS: 1.7 vs 6.4years, p<0.001 and OS: 5.2 vs 10.8years, p=0.001) and multivariate (PFS: HR=5.98, p<0.001 and OS: HR=2.99, p=0.048) analyses. These results were confirmed in an independent series of 58 grade II meningiomas (PFS: HR=7.22, p=0.002 and OS: HR=9.69, p=0.003). These associations and the integrated score could complement WHO grading.