Restored teeth can be used as samples for genotyping

The aim of this study is to warn that such samples should not be overlooked for a successful genotyping. Six restored dental pieces were randomly selected from extreme forensic situations. For each tooth two portions have been selected: the dentin of adjacent portions of the restoration area (DS) and the remaining root dentin (DR). DNA extraction was executed using the PrepFiler (TM) BTA kit according to the manufacturer's instructions and its quantification was realized with the Quantifiler (TM) Duo kit (both from Applied Biosystems). DS quantification values were comparatively higher than those of DR portion. These results may be related to a tertiary dentin formation, restorative and disorganized. The use of a sample with dentin tissue adjacent to a restoration area, covering the entire portion up to the pulp cavity, proved to be a good sample for genetic analysis, optimizing the quality of the sample volume. (C) 2015 Elsevier Ireland Ltd. All rights reserved.