Lack Of Thromboxane Synthase Prevents Hypertension And Fetal Growth Restriction After High Salt Treatment During Pregnancy

Preeclampsia (PE) is a potentially fatal pregnancy-related hypertensive disorder characterized by poor placenta development that can cause fetal growth restriction. PE-associated pathologies, including thrombosis, hypertension, and impaired placental development, may result from imbalances between thromboxane A(2) (TXA(2)) and prostacyclin. Low-dose aspirin, which selectively inhibits TXA(2) production, is used to prevent high-risk PE. However, the role of TXA(2) in aspirin-mediated protective effects in women with PE is not understood fully. In this study, we examined the role of prostanoids in PE using human samples and an induced PE mouse model. We demonstrated that the administration of salted drinking water (2.7% NaCl) to wild-type mice resulted in elevated placental TXA(2) synthase (TXAS) and plasma TXA(2), but not prostacyclin, levels, which was also found in our clinical PE placenta samples. The high salt-treated wild-type pregnant mice had shown unchanged maternal body weight, hypertension (MAP increase 15 mmHg), and decreased pup weight (similar to 50%) and size (similar to 24%), but these adverse effects were ameliorated in TXAS knockout (KO) mice. Moreover, increased expression of interleukin-1 beta and downstream phosphorylated-p38-mitogen-activated protein kinase were concordant with apoptosis induction in the placentas of salt water-treated wild-type mice. These alterations were not observed in TXAS KO mice. Together, our data suggest that TXA(2) depletion has anti-PE effects due to the prevention of hypertension and placental damage through downregulation of the interleukin-1 beta pathway.