Inactivation of microorganisms present in raw and pasteurized milk using antimicrobial photodynamic therapy

The aim of this study was to evaluate the effects of the antimicrobial photodynamic therapy with blue light and curcumin on the oral disinfection during 2h after treatment. Twenty-seven adults from randomized trial were randomly divided into groups (n=9): G PDT-treated with 30mg/L curcumin and blue LED (455±30nm)(400mW, 5min, 600mW/cm2), G Lonly with LED, G3 Conly with the drug. The saliva samples were collected for bacterial counts (colony forming units – CFU) at baseline and after the experimental phases (immediately, after 1 and 2h). The PDT group showed a significant reduction of CFU at immediately (5.71±0.48, p=0.001), after 1h (5.14±0.92, p=0.001) and 2h (5.35±0.76, p=0.001) compared with baseline. There were no significant changes for theLEDgroup. TheCgroupshowedasignificant increase of CFU after 1h (6.77±0.40, p=0.02) comparedwith baseline (5.57±0.91) and reduction after 2h (5.58±0.70) compared with post treatment (6.21±0.58). The PDT group showed significant difference in microbial reduction (p<0.05) compared to both light and curcumin groups until 2 h post-treatment. The new blueLED device for PDT using curcumin may be used for reduction of salivary microorganisms leading to overall disinfection of mouth.