The Preparation of Novel PEI Cleaning Metal Surface for Inhibiting the Proliferation and Migration of Eca109 Tumor Cells in Vitro

Event Abstract Back to Event The preparation of novel PEI cleaning metal surface for inhibiting the proliferation and migration of Eca109 tumor cells in vitro Kun Zhang1, Qinghua Li1, Yanting Zhang1, Xiangzhan Zhu1, Fangxia Guan1 and Jingan Li2 1 Zhengzhou University, School of Life Science, China 2 Southwest Jiaotong University, School of Material Science and Engineering, China Introduction: Esophageal cancer, the ninth most common malignancy, is the sixth cancer mortality causes [1],[2], and expandable stent placement is the most commonly performed treatments for malignant dysphagia [3]. However, the conventional stent cannot inhibit the tumour growth, due to most dip coatings of DESs are prepared with a limited dosage of the drug and a short period of drug delivery, and the exposed basements don't have the anti-tumor function [4],[5]. Herein, in order to combine the advantages of the immediate relief of esophageal dysphagia using stent with the longer-term benefits that are achieved through sustained and prolonged local inhibiting tumor growth, a novel PEI cleaning metal surface was prepared in vitro. Materials and methods: Polydopamine as a transitional layer was prepared on the stainless steel (SS) surface by the method of dipping in ultrasonic environment [6], and polyethyleneimine (PEI) was fabricated on the polydopamine modified surface for the better inhibiting tumor growth property. The morphologies and wettability of these samples were observed by SEM and the contact angle apparatus. Then, the proliferation of ECa109 cells was investigated using AO/PI reagent and CCK-8 kits, and the migration of ECa109 cells dyed with crystal violet was tested using a 24-well transwell system [7]. Results and discussions: Fig. 1 shows the schedule of fabricating PEI coating on the 317L SS (Fig.1 A), and the results of surface morphologies (Fig.1 B) and their wettability (Fig.1 C). The morphological changes of the surfaces showed that the surface of SS was characterized by non-uniform polishing scratches. On the SS-D sample, the polishing scratches disappeared and many micron and submicron particles appeared, while no other obvious change else happened after the deposition of PEI. The wettability result indicates that SS-D-P surface is more hydrophilic than SS surface and more hydrophobic than SS-D surface. Fig. 2 shows an obvious difference of humor cells proliferation after 6 h, 1d and 3d culture on the SS, SS-D and SS-D-P surface (Fig.2 A), and the cells seeded on SS and SS-D become polygonal at 1d, while cells on SS-D-P keep spherical morphology (Fig.2 B). In addition, there are more cells adhesion on the bottom of transwell with SS than that with SS-D-P (Fig.2 C). PEI was rich in amine groups, its toxicity is believed to be due to the charge interactions between these polymers and cell membrane, but also due to the interaction with serum proteins and causing aggregation that can lead to capillary embolism [8]. Therefore, these results demonstrated that SS-D-P could inhibit humor cell attachment, proliferation and migration by the deposition with PEI. Conclusions: In this work, the novel PEI cleaning metal surface has good property of inhibiting humor cells proliferation and migration, it has some reference value to the stent surface modification for treating malignant dysphagia in lumen cancer field of investigation. The joint talent cultivation funds of National Natural Science Foundation of China and Henan province (Grant no. U1504310; The financial support of National Natural Science Foundation of China (Grant no. 31170916#).References:[1] Chen MF, Yang YH, Lai CH, et al. Outcome of patients with esophageal cancer: a nationwide analysis. Annals of Surgical Oncology 2013; 20(9):3023-30.[2] Ajani JA, D'Amico TA, Almhanna K, et al. Esophageal and esophagogastric junction cancers, version 1.2015. Journal of the national comprehensive cancer network. 2015;13(2):194-227.[3] F.P. Vleggaar, P.D. Siersema. Expandable Stents for Malignant Esophageal Disease. Gastrointestinal Endoscopy Clinics of North America, 2011; 21(3): 377-88.[4] G. Li, Y. Chen, J. Hu, et al. A 5-fluorouracil-loaded polydioxanone weft-knitted stent for the treatment of colorectal cancer. Biomaterials, 2013; 34:9451-61.[5] Jieying Liu, Zhongmin Wang, Keqin Wu, et al. Paclitaxel or 5-fluorouracil/esophageal stent combinations as a novel approach for the treatment of esophageal cancer. Biomaterials. 2015; 53: 592-9.[6] H. Lee, S.M. Dellatore, W.M.P. Miller, B. Messersmith. Mussel-inspired surface chemistry for multifunctional coatings. Science, 2007; 318: 426-30.[7] Barden Chan, Paul A. VanderLaan, Vikas P. Sukhatme. 6-Phosphogluconate dehydrogenase regulates tumor cell migration in vitro by regulating receptor tyrosine kinase c-Met. Biochemical and Biophysical Research Communications. 2013; 439(2): 247-51.[8] Sushil K. Tripathi, Niharika Gupta, Manohar Mahato, et al. Selective blocking of primary amines in branched polyethylenimine with biocompatible ligand alleviates cytotoxicity and augments gene delivery efficacy in mammalian cells. Colloids and Surfaces B: Biointerfaces. 2014; 115: 79-85. Keywords: Cell Proliferation, Biocompatibility, Surface modification, bioactive interface Conference: 10th World Biomaterials Congress, Montréal, Canada, 17 May - 22 May, 2016. Presentation Type: Poster Topic: Surface and interfacial characterization Citation: Zhang K, Li Q, Zhang Y, Zhu X, Guan F and Li J (2016). The preparation of novel PEI cleaning metal surface for inhibiting the proliferation and migration of Eca109 tumor cells in vitro. Front. Bioeng. Biotechnol. Conference Abstract: 10th World Biomaterials Congress. doi: 10.3389/conf.FBIOE.2016.01.01314 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 27 Mar 2016; Published Online: 30 Mar 2016. Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Kun Zhang Qinghua Li Yanting Zhang Xiangzhan Zhu Fangxia Guan Jingan Li Google Kun Zhang Qinghua Li Yanting Zhang Xiangzhan Zhu Fangxia Guan Jingan Li Google Scholar Kun Zhang Qinghua Li Yanting Zhang Xiangzhan Zhu Fangxia Guan Jingan Li PubMed Kun Zhang Qinghua Li Yanting Zhang Xiangzhan Zhu Fangxia Guan Jingan Li Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.