Visualizing Cellular Metabolic Processes With Combined Nonlinear Optical Microscopy

Alba Alfonso Garcia,Timothy R. Smith,Rupsa Datta,Enrico Gratton,Eric O. Potma,Wendy F. Liu

Biomedical optics（2016）

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摘要

Macrophages are key players in inflammation. We combine label-free nonlinear optical microscopy techniques (coherent Raman scattering and fluorescence lifetime imaging) to link lipid and energy metabolism with macrophage phenotype in living cells.

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