Protective effect of total Flavonoid C-glycosides fromAbrusmollisextract on fatty liver induced by DL-Ethionine in mice

AIM:To study the effect of total flavonoid C-glycosides fromAbrus mollis extract(AME)on lipid dysregulation induced by Ethionine(DL-E).METHODS:ICR mice were ran-domly assigned to four groups(n=8):Control,DL-E,AME and AME+DL-E groups.Mice were gavaged with AME(200 mg/kg)for 7days.At day 7,mice of DL-E and AME+DL-E group were gavaged with DL-E(250 mg/kg),other two groups were treated with vehicle control(0.2% CMC-Na).Serum and liver tissues were collected 22hafter administration of DLE.The contents of hepatic lipids,serum lipids and ALT,AST levels were measured.The expressions of key genes involved in lipid metabolism were also observed by real-time PCR.RESULTS:The results indicated that AME provided significant protection against fatty liver by inhibiting the elevation of serum lipids,serum transaminase,reducing hepatic TG and TC accumula-tion,ameliorating the severity of pathological changes.Furthermore,AME significantly decreased DL-E-induced SREBP-1,FAS and ACC1high expressions,which resulting in the lipid homeostasis.The down-regulation of CPT-1αand PPARαthat reflect fatty acid metabolism induced by DL-E were reversed by AME treatment. CONCLUSION: The results indicated AME has hepatoprotective effect on Ethionineinduced fatty liver in mice and the effect was mostly due to the regulation on lipid synthesis and fatty acid metabolism.