In VitroRegeneration Studies in Wild Crucifer [Diplotaxis muralis(L.) DC.]

‘In vitro Regeneration Studies in Wild Crucifer’ were conducted in the Biotechnology Laboratory, Division of Plant Breeding and Genetics. MS medium supplemented with growth regulators at different concentrations was used in culturing of wild crucifer, Diplotaxis muralis (L.) DC. Aseptic seeds and survival of seedlings were observed after 1 and 4 weeks when treated with mercuric chloride (HgCl2) (0.1%) for 3 min. Considerable response was shown in 2,4-D 2.0 and 2.5 mg/l. No response was found in the MS medium supplemented with different concentrations of naphthaleneacetic acid (NAA). The MS medium with different concentrations of growth regulators showed a significant difference in the callus induction frequency and regeneration frequency for the genotype used. Coconut water used as organic adjuvant increased the callus induction and shoot regeneration efficiency. The present data indicate that 2,4-D 2.0 mg/l and 2.5 mg/l could be applied for maximum callus induction and 6-Benzylaminopurine (BAP) (5.0 mg/l) + 2,4-D (0.5 mg/l) for shoot regeneration.