The analysis for mitochondrial genome sequencing of Triplophysa lewanggensis

Triplophysa lewanggensis revealed that the complete length of its mitochondrial genome was 16,568 bp, composed of A (31.7%), T (27.1%), G (15.9%), C (25.4%), and A + T (58.8%). Its genetic constitution and arrangement were consistent with other Triplophysa, including 13 protein-coding genes, 22 tRNA genes, 2 rRNA genes, and 1 control area (D-loop). All genes were encoded by the H-strand, except for one protein-coding gene (ND6) and eight tRNA genes (tRNA(Gln), tRNA(Ala), tRNA(Cys), tRNA(Asn), tRNA(Tyr), tRNA(Ser(UCN)), tRNA(Glu), tRNA(Pro)) are encoded by the L chain, and the remaining genes are encoded by the H chain. The phylogenetic tree was divided into two main clades: one just including Triplophysa and the other including Oreonectes, Homatula, Schistura, Nemacheilus, Traccatichthys. T. lewanggensis is distributed in Southwest Guizhou Province attached to Yunnan-Guizhou Plateau, T. xiangxiensis is distributed in Hunan Province, they are near relatives, but their kinship with Triplophysa distributed in Tibetan Plateau is farther.