Electrochemical Studies and Potential Anticancer Activity in Ferrocene Derivatives

Several ferrocene derivatives (five mononuclear and two binuclear), including the new N-(p-chlorophenyl)-carboxamidoferrocene (1), were synthesized and their anticancer activity investigated. Two of them, 3 and 7, bearing a benzimidazole backbone were the most active against HeLa cells achieving IC50 values of similar to 5 mu M along with 4 with a dipyridylamine ligand (similar to 6 mu M). Complex 6, also with a benzimidazole backbone, displayed slightly higher values (similar to 11 mu M). Cyclic voltammetry studies show that while the non-cytotoxic ferrocene derivatives 1, 2, and 5 follow a ferrocene-based redox behavior, derivatives 3, 4, 6, and 7 exhibit a more complex mechanism. These complex mechanisms are consistent with a more effective cytotoxic activity. Mossbauer spectroscopy parameters reflect a very small influence of the substituents