Recent advances in high-throughput (13)C-fluxomics.

•13C-fluxomics provides quantification of metabolic activity in microorganisms.•Increasing flux analysis capacity has the potential to accelerate strain design.•HT 13C-fluxomics represents a trade-off between throughput and resolution.•HT workflows require automation, parallelization and integration of each step.•Recent advances may boost the emergence of integrated HT 13C-fluxomics workflows.