Study of electrochemical behavior of the Fe 3 O 4 nanoparticles in aprotic media

Peculiarities of electrochemical behavior of the Fe 3 O 4 magnetic nanoparticles immobilized on the surface of a platinum electrode in aprotic organic media were investigated. Possible scheme of electrochemical behavior of nanoparticles depending on pre-electrolysis potential (–1.3,–2.5 V) was suggested. The effect of pre-electrolysis time, potential scan rate and nature of supporting electrolyte on the processes investigated was determined. A linear dependence of electrochemical oxidation signal versus the concentration of nanoparticles in modifying suspension in the concentration range of 0.05—0.5 g L –1 was observed. The results of the performed research allow using magnetite nanoparticles as a direct signal-generating label in electrochemical immunoassay.