Screening some of plants from Silene Genus for 20-Hydroxyecdysone

Eight Silene species such as S. brachuica , S. guntensis, S. linicola , S. oreina, S. praemixta , S. pseudotites, S. viridiflora and S. wallichiana were screened for the main ecdysteroid 20-hydroxyecdysone by HPLC using the UV spectroscopy. HPLC analyses have shown all Silene plants except S. oreina contain 20-hydroxyecdysone, but in different concentration. Studies have shown that S. praemixta and S. viridiflora are rich phytoecdysteroids containing plants and the yields of total ecdysteroids are 2.0% and 1.6%, respectively. The results of investigation species of Silene : S. brachuica, S. praemixta, S. viridiflora, S. guntensis, S. linicola, S. pseudotites and S. wallichiana showed that the yields of 20E of these plants are 0.03, 0.27, 0.35, 0.082, 0.367, 0.071 and 0.08% respectively. The plants of S. linicola, S. praemixta and S. viridiflora suggested to be used for production of ecdysteroids containing preparations.