Implementation of an Ex Vivo TH17 Assay

Background: IL-17 secreting T-helper type 17 cells (Th17) have been shown to play a crucial role in mucosal immunity. Deficiency of Th17 cells is seen in primary immunodeficiency disorders (PID) such as autosomal dominant hyper IgE syndrome [STAT3 loss of function (LOF)], autosomal dominant chronic mucocutaneous candidiasis [STAT1 gain of function (GOF)] and IL12 and IL12RB1 deficiencies. Rapid and cost effective screening is required to identify these patients.