Secondary embryogenesis and plant regeneration in Psidium guajava L 41

With the objective of evaluating secondary somatic embryogenesis (SSE) and the germination of somatic embryos of guava (Psidium guajava L.) 2,4-dichlorophenoxyacetic acid (2,4-D) and N6-benzylaminopurine (6-BAP) were tested, respectively. Fresh weight (FW) and number of secondary somatic embryos formed (NSSEF) in the SSE, and the germination percentage (GP) were determined. The greatest value of NSSEF (48.5; P u003c 0.05) was obtained with 1 mg L-1 of 2,4-D, eight weeks after culture initiation. The highest GP (83%; P u003c 0.05) was reached with 0.25 mg L-1 of 6-BAP by culturing the SE in groups. These results suggested that somatic embryogenesis may be a possible alternative for the rapid propagation of promising guava tree selections.