Detection of L-alanylaminopeptidase activity in microorganisms using chromogenic self-immolative enzyme substrates
Bioorganic & Medicinal Chemistry(2017)
摘要
Three potential chromogenic enzymatic probes, each possessing a self-immolative spacer unit, were synthesised for the purpose of detecting l-alanylaminopeptidase activity in microorganisms. An Alizarin-based probe was the most effective, allowing several species to generate strongly coloured colonies in the presence of metal ions.
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关键词
Chromogenic enzyme substrates,l-Alanylaminopeptidase detection,Microorganisms
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