Assessment of mucosal integrity by quantifying neutrophil granulocyte influx in murine models of acute intestinal injury.

•Intestinal barrier malfunction is closely linked to disorders like IBD or GvHD.•Accurate assessment of barrier permeability is delicate.•FITC-dextran permeability analysis detects barrier breech with low sensitivity and specificity.•Mucosal neutrophil influx allows quantitative assessment of intestinal damage.•Mucosal neutrophil analysis adds sensitivity and specificity to combined approaches.