Convenient PCR method for variable-number tandem-repeat typing of Mycobacterium tuberculosis clinical isolates

Junji Seto,Takayuki Wada,Yu Suzuki,Tatsuya Ikeda,Katsumi Mizuta,Satoshi Mitarai,Tadayuki Ahiko

Journal of Microbiological Methods（2017）

引用 2|浏览15

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摘要

Variable-number tandem-repeat typing for Mycobacterium tuberculosis clinical isolates contributes to evidence-based tuberculosis control. However, cumbersome PCR procedures for the typing have disturbed routine analyses. We proposed a convenient PCR method for the typing using a PCR master mix that provides rapidity and long-term stability of the frozen PCR cocktail.

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关键词

Frozen PCR cocktail,High labor effectiveness,Human error reduction,Rapidity,SapphireAmp Fast Master Mix

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