In Vitro Activation of Cytotoxic T Lymphocytes By Muc1 PulsedDendritic Cells
The Open Cancer Immunology Journal(2009)
摘要
We studied antigen-specific and HLA-A2-restricted cytotoxic
activity against MUC1-positive ARH77 myeloma cell line in
vitro. A HLA-A2 specific MUC1-derived nonapetide
(139TSAPDTRPA147) was used as a tumor-associated antigen.
Myeloma-specific cytotoxic activity of MUC1-reactive CTL was
established by repeated stimulation of CTL via dendritic cells
loaded with MUC1-derived nonapeptide. We were able to
demonstrate that MUC1-reactive T cells can be identified and
expanded using a relatively simple in vitro techniques.
Antigen-specific stimulation via dendritic cells,
immunomagnetic sorting and rapid expansion of CTL without
loosing of their specific immune activity can be used
clinically even in poorly immunogenic diseases such as MM.
更多查看译文
AI 理解论文
溯源树
样例
生成溯源树,研究论文发展脉络
Chat Paper
正在生成论文摘要