Genetic Polymorphism of methylenetetrahydrofolate reductase (MTHFR) C 677 T and A1298C gene and risk of head and neck squamous cell carcinoma-A qualitative analysis

Background and Aim Functional polymorphisms in genes encoding enzymes involved in folate metabolism might modulate head and neck squamous cell carcinoma (HNSCC) risk of which Methylenetetrahydrofolate reductase gene (MTHFR) C677T and A1298C polymorphism is important due to changes in folate levels that can induce disorders in methylation pathway, which results in carcinogenesis. Materials u0026 Methods 25 samples of genomic DNA extracted from whole blood of both HNSCC cases and 25 age matched controls taken u0026 subjected to PCR amplification using specific primers followed by restriction fragment length polymorphism for detection of MTHFR C677T u0026 1298 C. Interactions with clinical histopathological parameters were also evaluated. Results 1298 AC/CC genotypes are associated with age ≥49 years, tobacco and alcohol (P Conclusion Variables like age ≥49 years, male gender, tobacco and alcohol were associated with MTHFR 1298AC or CC genotypes,indicating that individuals with these variables have higher risk. However further longitudinal studies are required to confirm these as established risk factors. No association between MTHFR C677T polymorphism and HNSCC was possible in this study.