Generation of Humanized Liver Mouse Model By Transplanted of Patient-Derived Fresh Human Hepatocytes.: Abstract# D2863

In the present, some research groups have produced immunodeficient mice with human liver tissue as a model system for the analysis of drug metabolism and liver regeneration. Mouse models are important for research and development of drugs and vaccines for viral infections. Recent progress in developing humanized mouse models permit studies of adaptive immune responses, innate host responses, and therapeutic approaches for several liver diseases of viral etiology. In this study, we generated a humanized liver mouse model by transplanted with fresh patient-derived hepatocytes (1 x 106 cells/mouse, intrasplenic injection) into pre-conditioned (50 mg/kg ganciclovir, intraperitoneal injection) TK-NOG mice. Successfully reconstitution of human hepatocytes in TK-NOG mouse liver tissues was observed with strong proliferation of human cells in a time-dependent manner, using cytokeratin 8/18 stain. Similarly, we detected significantly increased human albumin levels in TK-NOG mouse liver tissue and blood sera on immune staining and ELISA. Therefore, this humanized liver mouse model provides biomedical tool for studying human liver physiology, drug metabolism, and liver pathogenesis of viral etiology or liver regeneration.