Eleutherococcus Senticosus (Araliaceae) Leaf Morpho-Anatomy, Essential Oil Composition, And Its Biological Activity Against Aedes Aegypti (Diptera: Culicidae)

The roots of Eleutherococcus senticosus (Rupr. & Maxim.) Maxim., a well-known medicinal plant from Eastern Asia, are used worldwide for their known beneficial medicinal properties. Recently, the leaves have been used as an alternative to the roots. The present study was aimed at exploring the leaf essential oil as a potential source of compounds for mosquito management. Gas chromatography/mass spectrometry analysis of the leaf essential oil revealed 87 compounds, constituting 95.2% of the oil. alpha-Bisabolol (26.46%), beta-caryophyllene (7.45%), germacrene D (6.87%), beta-bisabolene (4.95%), and alpha-humulene (3.50%) were five of the major constituents. The essential oil was subjected to biting deterrence and repellent activity against mosquito Aedes aegypti. The biting deterrence of the oil produced a proportion not biting (PNB) value of 0.62 at 10 mg/cm(2) as compared with 0.86 of control DEET (N,N-diethyl-3-methylbenzamide) at a standard dose of 25 nmol/cm(2). Among individually selected compounds present in the oil (alpha-bisabolol, beta-caryophyllene, alpha-humulene, and caryophyllene oxide), only a-bisabolol produced a PNB value of 0.80, equivalent to DEET at 25 nmol/cm(2), whereas the others were not repellent. The artificialmixture (AMES-1) of these four selected compounds produced a relatively high PNB value of 0.80. The repellent activity measured by minimum effective dosage (MED) for alpha-bisabolol and alpha-humulene produced MED values of 0.094 and 0.104 mg/cm(2), respectively, as compared with 0.023 mg/cm(2) of DEET. The leaf essential oil, the artificial mixture (AMES-1), and other binary and tertiary combinations of major compounds showed no repellent activity. In addition, morpho-anatomical features of the leaf are provided for correct identification of the species.