URINARY OXALATE EXCRETION IN OBESE MOUSE MODEL

You have accessJournal of UrologyStone Disease: Basic Research & Pathophysiology II1 Apr 2018MP24-04 URINARY OXALATE EXCRETION IN OBESE MOUSE MODEL Kyle Wood, John Knight, Dean Assimos, Barbara Gower, and Ross Holmes Kyle WoodKyle Wood More articles by this author , John KnightJohn Knight More articles by this author , Dean AssimosDean Assimos More articles by this author , Barbara GowerBarbara Gower More articles by this author , and Ross HolmesRoss Holmes More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2018.02.757AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Studies have demonstrated the positive correlation between body weight/BMI and urinary oxalate excretion. We hypothesize that this is due to increased endogenous oxalate synthesis. This was studied in two models of obesity, ob+/ob+ mice and wild type mice fed a high fat diet. METHODS Wild type (WT) controls (n=5), high fat diet (HFD) (n=5), ob+/ob- lean controls (n=3), and ob+/ob+ (n=3) mice were fed a diet ultra-low in oxalate (<10µg/g diet) and glycolate (<3µg/g diet) and housed in metabolic cages. In the high fat diet, 45% of calories was fat vs 17% in normal diet. While on controlled diets the WT and HFD mice were administered a subcutaneous bolus of 2 µmoles 13C2-glycolate (oxalate precursor). 24 hour urine samples were collected. Ion chromatography coupled with mass spectroscopy was used to measure oxalate and glycolate isoptomers. RESULTS HFD mice weight compared to control was 42.1 g vs 30.5 g (p=0.0002). 24 hour urinary oxalate excretion indexed to urinary creatinine (Cr) was significantly higher in the ob+/ob+ mice and the HFD mice relative to the comparative control mice, 303.3 vs 95.6 µg/mg Cr (ob+/ob+ versus ob+/ob-),p=0.01 and 133.1 vs 106.2 µg/mg Cr (HFD versus WT), p=0.003. 24 hour urinary glycolate excretion was also significantly higher in each, 289.5 vs 105.0 µg/mg Cr (ob+/ob+ versus ob+/ob-),p=0.0001 and 193.1 vs 166.2 (HFD versus WT ), p=0.05. HFD mice produce more oxalate from glycolate following subcutaneous injection as measured by 13C2-oxalate levels, 1.5 vs 1.0 µg Cr, p=0.04. CONCLUSIONS These findings suggest that obesity increases endogenous oxalate synthesis. Further studies are needed to understand the metabolic changes that occur in obesity. © 2018FiguresReferencesRelatedDetails Volume 199Issue 4SApril 2018Page: e291 Advertisement Copyright & Permissions© 2018MetricsAuthor Information Kyle Wood More articles by this author John Knight More articles by this author Dean Assimos More articles by this author Barbara Gower More articles by this author Ross Holmes More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...