Abstract 17511: Microvesicles and MicroRNAs as Marker of Acute Kidney Injury After Cardiac Surgery in Children

Introduction: AKI is very common in paediatric cardiac surgery patients. The diagnosis relies on changes in serum creatinine and urine output, which lack specificity and sensitivity especially in newborns and premature infants. Diagnostic changes also occur late in the injury phase when interventions have limited effectiveness. Microvesicles (MV) are small particles (u003c1μm) in plasma that often contain miRNA. Their levels have been shown to be altered in animal models of AKI where they regulate injury responses. Hypothesis: MV and associated miRNA provide specific and early-detection markers for AKI and a tool to investigate AKI pathogenesis. Methods: Study subjects were 24 children (u003c17 yrs), undergoing cardiac surgery with cardiopulmonary bypass (CPB) without pre-existing inflammatory state, AKI or extracorporeal life support. AKI was diagnosed following the KDIGO definition. Blood and urine samples were collected pre-operatively and at 6 and 24 hours. Cytokines were measured on a MAGPIX platform. Leukoc...