Abiotic Stress Response in Yeast of a Type 1 Alternative Oxidase (lpaox1) from Lilium Pumilum
Molecular Microbiology Research(2018)
摘要
We cloned an LpAOX1 gene from Lilium puminlum , explored this gene expression in yeast under salts (NaCl, Na 2 CO 3 , NaHCO 3 ), pH value or oxidation (H 2 O 2 ) stress, the transgenic yeast growth better than untransgenic yeast in presence of stresses. The result reveal that LpAOX1 gene has effect on the yeast tolerance to the stresses and contribute to the elucidation of the LpAOX1 molecular mechanisms involved in the stress response pathway. LpAOX1 expression in the yeast control harmful ROS contents generation, alleviate the ROS induced oxidative stress and the resistance of transgenic yeast to salt stress was enhanced.
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关键词
Stress Response
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