Sequencing viral siRNAs to identify previously undescribed viruses and viroids in a panel of ornamental plant samples structured as a matrix of pools

Eric Verdin,Catherine Wipf-Scheibel, Patrick Gognalons,François Aller, Mireille Jacquemond,Mark Tepfer

Virus Research(2017)

引用 15|浏览13
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摘要
•Deep sequencing of siRNAs allowed de novo assembly of contigs to identify viruses.•Samples could be pooled before deep sequencing without loss of virus detection.•Detection of 27 previously unknown viruses, including all types of viral genomes.•Ornamental sector could be the source of novel plant diseases in all types of crops.
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关键词
Next-generation sequencing,siRNA,Virus,Viroid,Ornamental plant
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