Sample Preparation of Trypanosoma cruzi Surface, Extracellular, and Nuclear Subproteomes.

Carlos André Ornelas Ricart,Rayner Myr Lauterjung Queiroz,Agenor de Castro Moreira Dos Santos Júnior,Sébastien Charneau

Methods in molecular biology (Clifton, N.J.)（2019）

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摘要

The preparation of subproteome fractions prior to proteome analysis provides both the enrichment of proteins sub-represented in global proteome analysis and information on the cellular localization of the identified proteins. Here we describe protocols for the preparation of Trypanosoma cruzi surface and extracellular and nuclear fractions for further subproteome analysis.

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关键词

Biotinylation,Cell surface shaving,Nucleus,Plasma membrane,Proteome,Secretome

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