A platinum-porphine/poly(perfluoroether) film oxygen tension sensor for noninvasive local monitoring of cellular oxygen metabolism using phosphorescence lifetime imaging

•Phosphorescence lifetime imaging (PLIM)-based O2 level mapping is demonstrated.•The chemical inert, gas-permeable and bio-biocompatible property of PFPE enables more precise long-term cell study in vitro.•The easy fabrication of PFPE microfluidic chip enables on-chip study O2 environment of cell.