Parallel comparison of in situ Raman and NIR spectroscopies to simultaneously measure multiple variables toward real-time monitoring of CHO cell bioreactor cultures

•First comparison study of in situ NIR and Raman spectroscopies for CHO cell cultures monitoring.•NIR and Raman spectra were recorded in parallel in the same bioreactor cultures.•Six culture variables including antibody concentration were monitored simultaneously.•Multivariate figures of merit were established to compare the performances of models.