Bone regeneration of multichannel biphasic calcium phosphate granules supplemented with hyaluronic acid.

The present work is to investigate the efficiency of hyaluronic acid (HyA) supplemented biphasic calcium phosphate (BCP) injectable granule to promote the bone regeneration. The effect of adding HyA to the multi-channel BCP granule (MCG-HyA) was studied in terms of morphology, chemical structure, porosity, in-vitro and in-vivo biocompatibility, RT-PCR, western blot and compared with MCG. The addition of HyA to MCG successfully made the granules injectable type. In-vivo studies in rabbit model showed an enhancement in bone formation after 4 weeks of implantation and better handling characteristics for MCG-HyA than MCG. RT-PCR and Western Blotting studies revealed that MCG-HyA significantly unregulated the osteogenic gene and protein expressions respectively. Our results indicated that MCG-HyA could be used as a promising injectable bone substitute in clinical applications.