Bioactivity-guided identification of flavonoids with cholinesterase and β-amyloid peptide aggregation inhibitory effects from the seeds of Millettia pachycarpa.

•The anti-AD effects of different solvent extracts of M. pachycarpa were firstly reported.•An unusual oxidative ring-opening skeleton rotenoid and nine known flavonoids were obtained.•Four compounds showed strong inhibitory activities on cholinesterase.•Strong binding affinities of active compounds to cholinesterase were confirmed by fluorescence quenching.•Two compounds exhibited potent inhibitory effects against Aβ1-42 aggregation.