Quantitative Real-Time PCR with High-Throughput Automatable DNA Preparation for Molecular Screening of Nosema spp. in Antheraea pernyi.

Peipei Li,Rui Mi,Rui Zhao,Xiangcun Li,Bo Zhang,Dongmei Yue,Bo Ye,Zhenjun Zhao,Linmei Wang, Youmin Zhu, Chen Bao,Qi Fan,Xiaobin Jiang,Yaozhou Zhang

Journal of Invertebrate Pathology（2019）

引用 9|浏览21

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摘要

•High-throughput ALMS-qPCR method enhanced Nosema spp. detection.•DNA preparation of ALMS-qPCR is fast, automatable, and cost-effective.•Common PCR inhibitory factors were overcome in ALMS-qPCR.•The limit of detection (LOD) was 0.045 parasites/μL.•ALMS-qPCR is promising for routine pébrine screening and epidemiological studies.

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关键词

Nosema spp.,Diagnostic PCR,High-throughput,Automatable,Sensitive,Epidemiological studies

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