A thermostable chitinase from the antagonistic Chromobacterium violaceum that inhibits the development of phytopathogenic fungi.

Antônio J S Sousa,Christiana F B Silva,Jeanlex S Sousa,José E Monteiro,José E C Freire,Bruno L Sousa,Marina D P Lobo,Ana C O Monteiro-Moreira,Thalles B Grangeiro

Enzyme and Microbial Technology（2019）

引用 23|浏览24

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摘要

•A GH18 chitinase from Chromobacterium violaceum was produced in Escherichia coli.•The recombinant protein was purified and partially characterized.•The purified chitinase inhibited conidia germination of two Fusarium species.•A mechanism of action for the antifungal activity is suggested.

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关键词

Chitin,Glycoside hydrolase,Recombinant protein,Signal peptide,Heterologous expression,Antifungal activity

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