A systemic combined nontargeted and targeted LC-MS based metabolomic strategy of plasma and liver on pathology exploration of alpha-naphthylisothiocyanate induced cholestatic liver injury in mice.

•A combined targeted-nontargeted metabolomics strategy has been developed.•38 Potential plasma biomarkers and 17 potential liver biomarkers were found.•BA metabolism was obtained as the most important pathway.•CLI may correlate significantly with BA accumulation and related consequences.