Validation of quantitative real-time RT-PCR assays for the detection of six honeybee viruses.

Frank Schurr,Amandine Tison, Laura Militano, Nicolas Cheviron,Fabrice Sircoulomb,Marie-Pierre Rivière,Magali Ribière-Chabert,Richard Thiéry,Eric Dubois

Journal of Virological Methods（2019）

引用 25|浏览18

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摘要

•The accuracy of 5 RT-qPCRs was assessed for their ability to quantify Europe’s most frequently-detected viruses.•Honeybees collected in France (2012–2019) were tested to determine the frequency of each viral load.•Viral load thresholds were suggested to differentiate covert from overt infections.

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关键词

Quantitative RT-PCR,Fluorogenic probe,Trueness,Precision,Diagnostic threshold

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