Identification of Carassius auratus gibelio liver cell proteins interacting with the GABA A receptor γ2 subunit using a yeast two-hybrid system

The γ-aminobutyric acid type A (GABA A ) receptor is an important pentameric inhibitory neurotransmitter receptor, and the γ2 subunit of this receptor plays a key role in potentiation of the GABA A response. We previously detected that the expression of GABA A receptor in the livers of Carassius auratus gibelio significantly increased after medication (avermectin and difloxacin treatment). In order to better understand the mechanism of action of the GABA A receptor γ2 subunit in the livers of C. auratus gibelio , we constructed a C. auratus gibelio liver cDNA library (the titer value of 1.2 × 10 6 cfu/mL) and identified the proteins that interact with the GABA A receptor γ2 subunit by using a yeast two-hybrid assay. The yeast two-hybrid screening yielded seven positive clones, namely, prelid3b, cdc42, sgk1, spg21, proteasome, chia.5, and AP-3 complex subunit beta-1, all of which have been annotated by the NCBI database. The functions of these proteins are complex; therefore, additional studies are required to determine the specific interactions of these proteins with the GABA A receptor γ2 subunit in the liver of C. auratus gibelio . Although the interactions identified by the yeast two-hybrid system should be considered as preliminary results, the findings of this study may provide further direction and a foundation for future research focusing on the mechanisms of the GABA A receptor γ2 subunit in C. auratus gibelio livers.