Genome-wide evidences of bisphenol a toxicity using Schizosaccharomyces pombe

Archives of Pharmacal Research(2018)

引用 4|浏览6
暂无评分
摘要
To clarify reliable toxic mechanisms of bisphenol A (BPA), an endocrine disrupting chemical, we approached an alternative animal and whole genome analyses with the yeast knockout library (YKO) of Schizosaccharomyces pombe . As results, the 50% growth inhibition concentrations (GI 50 ) of BPA was approximately 600 μM and the YKO—three step screening revealed the top 10 target candidate genes including dbp2, utp18, srs1, tif224, use1, qcr1, etc. The screening results were confirmed in human embryonic stem cell (hES)-derived hepatic cells and HepG2 human liver cancer cells. We found BPA down-regulated UQCRC, the human orthlog of S. pombe - qcr1, as a part of the mitochondrial respiratory chain, in HepG2 cells and hESs during cell differentiation into hepatic cells. Therefore, BPA may induce mitochondrial dysfunction and disruption of differentiation by suppressing UQCRC1 .
更多
查看译文
关键词
Bisphenol A, Differentiation, UQCRC1, Genome, Mitochondria, Yeast
AI 理解论文
溯源树
样例
生成溯源树,研究论文发展脉络
Chat Paper
正在生成论文摘要