Fluorescent Bead–DNA Conjugate‐based Dual Signal Amplification Technology
BULLETIN OF THE KOREAN CHEMICAL SOCIETY(2016)
摘要
One of the major challenges in DNA microarrays for their widespread application is assay sensitivity. Therefore, to achieve higher sensitivity, we developed a dual signal amplification technology based on the fluorescence beads (FB). It is the first report of the development of a dual signal amplification technology for DNA microarrays by synthesizing and applying the FB-DNA conjugates. The FB-DNA conjugates and Cy5-T1-FB-mixed-DNA demonstrated similar to 250-325-fold increase in sensitivity when compared with the conventional use of Cy5-labeled oligonucleotides such as Cy5-T1. Furthermore, the Cy5-T1-FB-mixed-DNA demonstrated similar to 5-1.3-fold increase in the sensitivity when compared with that of the FB-DNA conjugates alone.
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关键词
Microarray,Fluosphere,Fluoro-microbeads,Signal amplification,9G DNA chips
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