Poly-Gamma-Glutamic Acid Coupled Pseudomonas Putida Cells Surface-Displaying Metallothioneins: Composited Copper(Ii) Biosorption And Inducible Flocculation In Aqueous Solution

Poly-gamma-glutamic acid (gamma-PGA) is a biodegradable macromolecular polymer that is capable of adsorbing heavy metal ions through its side chains. In this study, a new biocomposite biosorbent that incorporates Cu2+ adsorption and recovery from aqueous solutions was developed through immobilization of a glutamine-binding protein GlnBP and cyanobacterial metallothionein SmtA onto the surface of Pseudomonas putida cells using an InaQ-N protein anchor, followed by coupling the cells with side chain-activated gamma-PGA. The surface localization of the fusion protein (InaQ)(2)-GlnBP-(SmtA)(2) was confirmed by SDS-PAGE/Western blot, immunofluorescence microscopy and fluorescence-activated cell sorting assays. The engineered biocomposite MB546-PGA was capable of Cu2+ adsorption across a pH range of 3.5-5.5 and a relatively broad temperature range of 20-60 degrees C, with a maximum adsorption capacity for Cu2+ of 145.99 mg g(-1). The pseudo-second-order equation was applicable to the sorption data. Moreover, MB546-PGA exhibited an inducible flocculation capability following Cu2+ adsorption. The infrared spectroscopy data showed that the hydroxyl and amino groups of MB546-PGA were involved in Cu2+ adsorption, and gamma-PGA likely bound to the cells through its carbonyl and amino groups. Given the features of the high capacity of Cu2+ adsorption and the easily inducible flocculation, the developed biosorbent shows potential for use in the remediation and purification of polluted or waste waters.