α-Eleostearic acid-containing triglycerides for a continuous assay to determine lipase sn-1 and sn-3 regio-preference

•Direct and continuous spectrophotometric method to screen lipases regio-selectivity.•Tailor-made triglycerides containing α-eleostearic acid are used as substrates.•Could determine directly after lipolysis the regio-selectivity of various lipases.•Lipase assay compatible with high throughput screening.