Fundamental Study of Improving an Efficient Assay in Patient of Drug-induced Allergy

The drug-induced lymphocyte stimulation test (DLST) and the cell migration analysis (CMA) are widely used as in vitro techniques to identify medicine causing drug-induced allergy (DIA). Unfortunately, however, the positive rate of these tests for identifying the causative drug in DIA is insufficiently high. In this study, we tried to establish a simple and rapid method with Jurkat cells and patients' serum to screen DIA before confirming by DLST and CMA. The sera were obtained from the healthy volunteer and the patients who had allergy-like symptoms and CMA. The real-time recording of cell mobility was performed up to 4 h using an optical accessible chmotaxis apparatus with Jurkat cells and patients' serum. When the kinetics of Jurkat cells were analyzed using the images of cell migration and the ImageJ software, the significant chemotaxis of Jurkat cells was observed against the sera of patients who had the drug for DIA identified by CMA. The positive rate of CMA rose from 16.7% to 50% after employing this rapid and beforehand method for diagnosing DIA followed by selecting patients' serum for the inspection. These results suggest that it may be an effective method for analyzing the kinetics of Jurkat cells with patients' serum before CMA for diagnosing the possibility of DIA.