In Vitro Seed Germination And Morphophysiological Parameters Of Tabernaemontana Catharinensis A. Dc. Microcuttings

The aim of this study was to evaluate in vitro seed germination and the morphophysiological parameters of Tabernaemontana catharinensis microcuttings. In the germination bioassays, the seeds were immersed at solutions of 0.0; 300; and 600 mg L-1 GA(3) for 24 or 48h. The seedlings from in vitro germination were used to produce microcuttings, which were inoculated in MS growth medium supplemented with 0.0; 1.0; 2.0; and 4.0 mg L-1 of IBA. The use of GA(3) did not lead to higher seed germination rates in T. catharinensis and the germination rate was negatively affected in seeds exposed to GA(3) for longer periods (48h). Rhizogenesis of T. catharinensis microcuttings can be induced without the need for phytoregulators. However, the morphophysiological parameters can be improved with the addition of IBA to the growth medium, showing that the use of IBA potentiates the production of aseptic microcuttings of T. catharinensis.