Ultra-High Sensitive Cardiac Troponin I Baseline Levels Are Affected By Age And Sex

Introduction and Hypothesis: Relations between elevated circulating cardiac troponin I (cTnI) levels and adverse cardiac outcomes were established prior to the advent of methods to measure extremely low levels of cTnI. Recently developed ELISA assays/platforms with ultra-high sensitivity cTnI (u-hs-cTnI) allow accurate measurement in healthy subjects. We hypothesized that women have lower u-hs-cTnI levels than their male counterparts, and that levels would also differ by age. Methods: Two healthy cohorts (200 women, 200 men) aged 18-97 years were analyzed in duplicate using the Quanterix cTnI assay on the Simoa HD-1 Analyzer. The u-hs-cTnI 99th percentiles were calculated as the upper limits considering a robust estimation against outliers with 90% confidence intervals Results and Discussion: The LLOD of u-hs-cTnI assay was calculated to be 0.005pg/ml; we accurately quantified u-hs-cTnI in 93% of healthy individuals. The combined 99th percentile was calculated to be 7.94 pg/ml (90% Confidence Interval [CI...